欧宝在线登录

教师名录

欧宝在线登录:蔡冬波

作者:欧宝在线登录发布时间:2021年06月18日 10:15

一、基本信息

蔡冬波,博士,讲师,硕士生导师

电子邮箱:caidongbo(AT)hubu.edu.cn

研究方向:芽胞杆菌代谢工程育种;芽胞杆菌异源蛋白高效表达体系

二、教育背景

2015.9–2018.6  欧宝在线登录,生物化学与分子生物学,博士

2012.9–2015.6  华中农业大学,发酵工程,硕士

2009.9–2013.6  华中农业大学,国家生物技术理科人才培养基地班应用生物技术专业,学士(本硕连读)

三、工作经历

2020.07至今   欧宝在线登录,讲师

2018.7–2020.6  欧宝在线登录,博士后

四、科研项目

1.    地衣芽胞杆菌转录因子PhoP调控聚γ-谷氨酸合成酶PgsBCAA机制研究,湖北省自然科学基金青年项目,2019-2021,2019CFB319,3万,主持,在研

2.    烤烟根际微生物资源利用及健康调控技术研究,中国烟草总公司湖北省分公司,2020-2022,027Y2020-013,30万,主持,在研

3.    地衣芽胞杆菌转录因子PhoP调控聚γ-谷氨酸合成代谢机制,中国博士后科学基金面上资助,2018-2020,2018M642802,5万,主持,结题

4.    地衣芽胞杆菌转录因子PhoP调控聚γ-谷氨酸合成酶基因簇pgsBCAA的机制研究,湖北省博士后科技活动择优资助,2018-2020,Z31,3万,主持,结题

五、发表论文

1.      Cai D, Zhu J, Zhu S, Lu Y, Zhang B, Lu K, Li J, Ma X, Chen S*. Metabolic engineering of main transcription factors in carbon, nitrogen, and phosphorus metabolisms for enhanced production of bacitracin in Bacillus licheniformis. ACS Synth Biol, 2019 19;8(4):866-875. (SCI 2 IF:5.571)

2.      Cai D, Zhang B, Zhu J, Xu H, Liu P, Wang Z, Li J, Yang Z*, Ma X, Chen S*. Enhanced bacitracin production by systematically engineering S-Adenosylmethionine Supply Modules in Bacillus licheniformis. Front Bioeng Biotechnol, 2020, 8:305 (SCI 2 IF:5.122)

3.      Cai D, Chen Y, He P, Wang S, Mo Fei, Li X, Wang Q, Nomura CT, Wen Z, Ma X*, Chen S*. Enhanced production of Poly-γ-glutamic acid by improving ATP supply in metabolically engineered Bacillus licheniformis. Biotechnol Bioeng, 2018,115(10):2541-2553 (SCI 2 IF:4.26)

4.      Cai D, Zhang B, Rao Y, Li L, Zhu J, Li J, Ma X, Chen S*. Improving the utilization rate of soybean meal for efficient production of bacitracin and heterologous proteins in the aprA-deficient strain of Bacillus licheniformis. Appl Microbiol Biotechnol. 2019;103(12):4789-4799 (SCI 2Top, IF: 3.67)

5.      Wu F, Cai D*, Li L, Li Y, Yang H, Li J, Ma X, Chen S*. Modular metabolic engineering of lysine supply for enhanced production of bacitracin in Bacillus licheniformis. Appl Microbiol Biotechnol, 2019, 103(21-22):8799-8812 (SCI 2Top, IF:3.67)

6.      Cai D#, Wang H#, He P, Zhu C, Wang Q, Wei X, Nomura CT, Chen S*. A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis. Microb Cell Fact, 2017,16(1):70 (SCI 2 IF:4.402)

7.      Cai D, He P, Lu X, Zhu C, Zhu J, Zhan Y, Wang Q, Wen Z, Chen S*. A novel approach to improve poly-gamma-glutamic acid production by NADPH Regeneration in Bacillus licheniformis WX-02. Sci Rep, 2017, 7:43404 (SCI 3 IF:4.011)

8.      Cai D, Wei X, Qiu Y, Chen Y, Chen J, Wen Z, Chen S*. High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase. J Appl Microbiol, 2016, 121(3):704-12 (SCI 3 IF:2.683)

9.      Cai D, Rao Y, Zhan Y, Wang Q, Chen S*. Engineering Bacillus for efficient production of heterologous protein: current progress, challenge and prospect. J Appl Microbiol, 2019, 126 (6), 1632-1642 (SCI 3 IF:2.683)

10.   Cai D, Zhu C, Chen S*. Microbial production of nattokinase: current progress, challenge and prospect. World J Microbiol Biotechnol, 2017,33(5):84 (SCI 3 IF:2.652)

11.   Cai D, Hu S, Chen Y, Liu L, Yang S, Ma X, Chen S*. Enhanced production of Poly-γ-glutamic acid by overexpression of the global anaerobic regulator Fnr in Bacillus licheniformis WX-02, Appl Biotechnol Biochem, 2018, 185(4):958-970 (SCI 3 IF:2.14)

12.   Cai D, Liu M, Wei X, Li X, Wang Q, Nomura CT, Chen S*. Use of Bacillus amyloliquefaciens HZ-12 for High-Level Production of the Blood Glucose Lowering Compound, 1-Deoxynojirimycin (DNJ), and Nutraceutical Enriched Soybeans via Fermentation. Appl Biotechnol Biochem, 2016,181(3):1108-1122  (SCI 3 IF:2.14)

13.   Cai D, Zhu J, Li Y, Li L, Zhang M, Wang Z, Yang H, Li J, Yang Z, Chen S*. Systematic engineering of branch chain amino acid supply modules for the enhanced production of bacitracin from Bacillus licheniformis. Metab Eng Co, 2020. Jun 11;11:e00136.

14.   Zhu J#, Cai D#, Xu H, Liu Z, Zhang B, Wu F, Li J, Chen S*. Enhancement of precursor amino acid supplies for improving bacitracin production by activation of branched chain amino acid transporter BrnQ and deletion of its regulator gene lrp in Bacillus licheniformis. Synth Syst Biotechnol, 2018, 236-243.

六、授权专利

1.      陈守文,蔡冬波,陆兴澄,王勤,陈杨阳,何鹏辉。一株可以高产聚γ-谷氨酸的地衣芽胞杆菌工程菌,专利号:ZL201611110074.0

2.      陈守文,蔡冬波,师璐,李顺意,胡施颖,杨勇。一种可以提高芽胞杆菌生物量的基因工程改造方法,专利号:ZL201610821676.6

3.      陈守文,蔡冬波,魏雪团,周银华,陈敬帮,祁高富,冀志霞。一株可以高效分泌纳豆激酶的地衣芽胞杆菌工程菌,专利号:ZL201410417443.0

4.      陈守文,朱江,蔡冬波,李俊辉,段茂华,楼丽君,陈晓斌。强化YvbW表达的地衣芽胞杆菌在杆菌肽生产中的应用,专利号:ZL201810246295.9

5.      陈守文,吴非,蔡冬波,张博闻,李俊辉,楼丽君,陈晓斌。敲除malR的地衣芽胞杆菌菌株在杆菌肽生产中的应用,专利号:ZL201810898060.8

6.      陈守文,何鹏辉,彭炳,蔡冬波,陈耀中,王勤,魏雪团,一种基于核糖体结合位点改造的启动子优化方法,中国发明专利号:ZL201710062695.X

7.      陈守文,许玉享,蔡冬波,杨勇,高林,王瑞,高加明,4-氨基丁酸氨基转移酶在提高伊枯草菌素A发酵产量中的应用,ZL201910685428.7

8.      陈守文,王豪,蔡冬波,何鹏辉,朱江,魏雪团,朱程军,一种提高地衣芽胞杆菌外源蛋白分泌水平的方法,中国发明专利号:ZL201710052525.3

9.      陈守文,蔡冬波,杨帆,张清,马昕,陈建刚,地衣芽胞杆菌谷氨酸脱氢酶突变体S277W在聚γ-谷氨酸合成中的应用,中国发明专利号:ZL201911115629.X

10.   陈守文,杨帆,蔡冬波,陈耀中,张清,马昕,陈建刚,消化链球菌谷氨酸脱氢酶GdhA在提高地衣芽胞杆菌聚γ-谷氨酸产量中的应用,中国发明专利号:ZL201911236047.7

欧宝在线登录(中国)控股公司